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Vimentin 抗体

This anti-Vimentin antibody is a 小鼠 单克隆 antibody detecting Vimentin in WB, IHC, FACS, IHC (p), ICC 和 IHC (fro). Suitable for 人, 大鼠 和 小鸡.
产品编号 ABIN204914
发货至: 中国

Quick Overview for Vimentin 抗体 (ABIN204914)

抗原

See all Vimentin (VIM) 抗体
Vimentin (VIM)

适用

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人, 大鼠, 小鸡

宿主

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小鼠

克隆类型

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单克隆

标记

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This Vimentin antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • 特异性

    Mesenchymal cells and mesenchymal derived tumors e. g. lymphoma, sarcoma and melanoma. Reacts with the 57kD protein corresponding to vimentin. Species cross-reactivity: Human, rat, chicken, potoroo.

    纯化方法

    Protein G purified

    免疫原

    Vimentin.

    Type of Immunogen: Purified protein

    亚型

    IgG1
  • 应用备注

    Approved: Flo, ICC, IHC, IHC-Fr, IHC-P, WB (1:100 - 1:1000)

    Usage: Suitable for use in Immunohistochemistry, Flow Cytometry, Western Blot and Immunocytochemistry. Immunohistochemistry (Frozen, Paraffin): 1:25-1:200 using ABC method. Paraffin sections require HIER with 10 mM citrate buffer,  pH 6.0, for 10 min. Cool sections at room temperature for 20 min. Flow Cytometry: 1-25-1:200. Western Blot: 1:100-1:1000. Optimum dilutions to be determined by researcher. Hybridoma: PAI mouse myeloma cells with spleen cells from a BALB/c mouse immunized with Vimentin isolated from porcine lens.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS,  pH 7.2, 0.1 % sodium azide. No stabilizing proteins added.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C,-20 °C

    储存方法

    4°C or -20°C, Avoid freeze-thaw cycles.
  • 抗原

    Vimentin (VIM)

    别名

    Vimentin

    背景

    Name/Gene ID: VIM

    Synonyms: VIM, Vimentin

    基因ID

    7431

    UniProt

    P08670

    途径

    Caspase Cascade in Apoptosis
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